FIG. 9.

AML1-ETO down-regulates the expression of C/EBPα, CDK4, and c-myc. (A) Total RNA (10 μg) was prepared from Kasumi-1 cells and U937T cells and their derived clones after they had been cultured in the presence or the absence of tetracycline for 72 h, electrophoresed, blotted, and hybridized with α-³²P-labeled C/EBPα cDNA. (B) Time course of the expression of C/EBPα, CDK4, and c-myc upon tetracycline withdrawal. (C) Effects of TPA on the level of C/EBPα mRNA. The cells were treated with 65 nM TPA for 48 h starting 24 h after induction of AML1-ETO. The 28S rRNA is shown to show relative RNA loading in the gel.