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. 2021 Dec 4;11(12):3779–3790. doi: 10.1016/j.apsb.2021.11.022

Figure 4.

Figure 4

PEG-ASNase- and cycloheximide-induced lipolysis is associated with phosphorylation of eIF2α. (A) The NEFA concentrations in the conditioned media of differentiated 3T3-L1 cells were elevated after PEG-ASNase (0.1 U/mL) and cycloheximide (CHX, 50 μg/mL) treatment. The protein levels of p-eIF2α, p-PKA, ATGL, and p-HSL in (B) WAT and (C) differentiated 3T3-L1 adipocytes were upregulated after PEG-ASNase. (D) Treating differentiated 3T3-L1 cells with cycloheximide leads to a similar increase in ATGL, p-HSL, p-eIF2α, and p-PKA as PEG-ASNase. Data is the mean ± SD with statistical significance indicated as ∗∗∗∗ for P < 0.0001. Data in (A) representative of 3 independent experiments.