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. 2021 Aug 17;11(12):3950–3965. doi: 10.1016/j.apsb.2021.07.027

Figure 1.

Figure 1

Bioengineered miR-124-3p selectively controls the levels of many proteins involved in cellular components critical to the metastatic potential of cells. (A) Volcano plots of significantly-altered proteins (FC>30% and FDR<0.3) in A549 cells treated with hBERA/miR-124-3p versus control LSA, as well as LSA versus vehicle. 147 proteins were downregulated by hBERA/miR-124-3p, among which some targets are reported and many are not verified yet. Further, control RNA showed minimal impact on cell proteome, compared with vehicle treatment. n = 3/group. (B) Heatmap of the top 60 most downregulated proteins by hBERA/miR-124-3p, compared to LSA or vehicle treatments. (C) Specificity of hBERA/miR-124-3p in the regulation of miR-124-3p targeted genes is supported by miRNA enrichment analyses, which readily identified hsa-miR-124-3p underlying genes encoding the proteins significantly downregulated by hBERA/miR-124-3p. (D) Major biological processes were revealed by Gene Ontology (GO) enrichment analysis of downregulated proteins.