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. 2021 Jun 16;11(12):3966–3982. doi: 10.1016/j.apsb.2021.06.007

Figure 2.

Figure 2

Magnolol promotes the degradation of multiple mitochondrial proteins via mitophagy. (A) SH-SY5Y cells were treated with magnolol 100 μmol/L for indicated hours. Whole-cell lysates were analyzed for outer mitochondrial membrane (OMM) proteins [mitofusion1 (MFN1), mitofusion2 (MFN2), and Tom20] and inner mitochondrial membrane (IMM) protein (Tim23) by immunoblotting, and actin was used as control. (B) Quantification of mitochondrial proteins degradation after magnolol 100 μmol/L treatment for 24 h in SH-SY5Y cells. Data are presented as mean ± SD (n = 3). ∗∗∗P < 0.001 (two-way ANOVA). (C) SH-SY5Y cells were treated with magnolol 100 μmol/L for 24 h. Whole-cell lysates were analyzed for mtDNA encoded protein COX II and actin by immunoblotting. (D) Quantification of COX II from (C). Data are presented as mean ± SD (n = 3). ∗∗P < 0.01 (Student's t-test). (E) SH-SY5Y cells were treated with magnolol 100 μmol/L for 24 h. Whole-cell lysates were analyzed for mitochondrial matrix protein HSP60 and actin by immunoblotting. (F) Quantification of HSP60 from (E). Data are presented as mean ± SD (n = 3). ∗P < 0.05 (Student's t-test).