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. 2021 Jun 16;11(12):3966–3982. doi: 10.1016/j.apsb.2021.06.007

Figure 3.

Figure 3

Magnolol-induced mitophagy requires PINK1 and Parkin expression. (A) SH-SY5Y cells were treated with magnolol 100 μmol/L for indicated hours. Whole-cell lysates were analyzed for PINK1 by immunoblotting. (B) SH-SY5Y cells were transfected with control (Scr) siRNA and PINK1 siRNA for 48 h and then treated with magnolol 100 μmol/L for 24 h. Immunoblotting for mitochondrial proteins was performed as indicated. (C) Quantification of mitochondrial proteins degradation in (B). Data are presented as mean ± SD (n = 3). ∗∗∗P < 0.001 (two-way ANOVA). (D) SH-SY5Y cells were transfected with Scr siRNA and Parkin siRNA for 48 h and then treated with magnolol 100 μmol/L for 24 h. Immunoblotting for mitochondrial proteins was performed as indicated. (E) Quantification of mitochondrial proteins degradation in (D). Data are presented as mean ± SD (n = 3). ∗∗∗P < 0.001 (two-way ANOVA). (F) SH-SY5Y cells were pretreated with Ac220 10 μmol/L for 2 h and then treated with magnolol 100 μmol/L and magnolol plus Ac220 for 24 h. Immunoblotting for mitochondrial proteins and PINK1 was performed as indicated. (G) Quantification of PINK1 from (F). Data are presented as mean ± SD (n = 3). ∗∗P < 0.01 (one-way ANOVA). N.S, no significant difference. (H) Quantification of mitochondrial proteins degradation in (F). Data are presented as mean ± SD (n = 3). ∗∗∗P < 0.001 (two-way ANOVA).