Fig. 3.

H19 can regulate autophagy and ferroptosis. (A) The relative H19 RNA level was measured via qRT‐PCR after MCF7 cells were treated with erastin (20 μm) for 48 h (n = 3, *P < 0.05, t‐test; values represent the mean ± SD). (B) MCF7 cells were treated with erastin (20 μm) for 48 h. Beclin1 and LC3 protein expression was measured via western blotting. (C) MCF7 cells were subjected to knockdown of H19. The relative lipid ROS levels were assayed via C11‐BODIPY fluorescence. (D) MCF7 cells were subjected to knockdown of H19. The level of autophagy was measured by assessing Cyto‐ID green fluorescence. (E) MCF7 cells were treated with or without rapamycin after knocking down H19. The relative lipid ROS levels were assayed via C11‐BODIPY fluorescence.