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. 2021 Nov 25;12(1):jkab393. doi: 10.1093/g3journal/jkab393

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© The Author(s) 2021. Published by Oxford University Press on behalf of Genetics Society of America.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Padh1 promoter-expressed OsTIR1 is more effective than F-box proteins used in the Kanke system. (A) Schematic of the genome-integrated cassettes expressing F-box proteins (not drawn to scale). The Padh1-OsTIR1 cassette expressing codon-optimized OsTIR1 from the strong Padh1 promoter was designed and constructed in this study. The Padh15-skp1-AtTIR1-NLS cassette and the Padh15-double-skp1-TIR1 cassette were developed by Kanke et al. (2011). (B) Spot assay showed that growth inhibition occurred at a lower concentration of NAA when the mcm4-IAA17 allele was combined with the Padh1-OsTIR1 cassette than when combined with the Padh15-skp1-AtTIR1-NLS cassette or the Padh15-double-skp1-TIR1 cassette.