Table 2.
Our improved AID system is effective on genes that are refractory to the Kanke system
| Essential gene | Results obtained using the Kanke system (according to Kanke et al.) | Results obtained using our improved AID system (the concentration of 5-adamantyl-IAA that resulted in complete growth inhibition)a |
|---|---|---|
| mcm4 | Severe growth defect | Complete growth inhibition at 1 nM |
| orc2 | Severe growth defect | Complete growth inhibition at 10 nM |
| cdc45 | Slow growth | Complete growth inhibition at 10 nM |
| orc6 | Slow growth | Complete growth inhibition at 10 nM |
| pol1 | No effect | Complete growth inhibition at 10 nM |
| cdc20 | No effect | Complete growth inhibition at 100 nM |
| mcm10 | No effect | Complete growth inhibition at 100 nM |
| ssl3 | No effect | Complete growth inhibition at 100 nM |
a
Growth phenotype was examined by spot assays using PMG plates containing 4 different concentrations of 5-adamantyl-IAA (0.1, 1, 10, and 100 nM) at 30°C.