FIG. 5.
Neo-PAP and classic PAP appear to be differentially phosphorylated. Western blotting with an anti-HA epitope antibody was performed on extracts of 293 cells that were not transfected (“none”) or transfected with plasmids encoding green fluorescent protein (GFP), HA-PAP II, or HA-neoPAP. Some lysates (indicated with a plus sign) were treated with potato acid phosphatase prior to loading onto SDS–4 to 20% polyacrylamide gel electrophoresis. Cell equivalents were 1.6 × 105 per lane. Results are representative of three separate experiments.