Table 1.

Major improvements of tsRFun compared to tRF2Cancer

Data features/functionalities	tRF2Cancer	tsRFun
Clear/clash data	None	Yes
CLIP data	None	Yes
Small RNA seq data format	FASTA	FASTA/FASTQ
tsRNA identification	tRFs (‘tRFfinder’)	tRFs and tiRNAs (‘tsRFinder’)
tsRNA pan-cancer analysis	None	Yes
tsRNA targets prediction	None	Yes (‘tsRTarget’)
tsRNA expression profile	1000 tRFs	∼5000 tsRNAs
tsRNA and miRNA competition relationships	None	Yes
tsRNA Function enrichment	None	15types of gene sets (‘tsRFunction’)