FIG. 2.

Detection of Irr mRNA by RT-PCR in kidney extracts. RT-PCR analysis was performed on mRNA isolated from kidney of WT and Irr^−/− mice using a set of primers in the Irr sequence, as described in the text. The size of the expected Irr PCR product is indicated. The upper band corresponds to Ir. The identity of the two bands was determined by sequence analysis. No PCR product was detected when the reverse transcription step was omitted (data not shown). Lane 1, molecular size markers; lane 2, Irr^−/− mice: lane 3, WT mice.