Figure 2.

ZFAS1 promoted the CRC cell malignant phenotype in vitro

(A) The expression of ZFAS1 in colorectal cancer cell lines (SW480, RKO, HT-29, HCT116) and normal colonic epithelial cells (FHC) was detected by qRT-PCR. (B) Two specific siRNAs were transfected into SW480 and RKO cell lines and effectively downregulated the expression of ZFAS1 compared with the negative control (Si-NC). (C) The CCK-8 assay showed that knockdown of ZFAS1 inhibited the growth of SW480 and RKO cell lines. (D) The colony formation assay showed that the colony numbers of the si-ZFAS1 groups were less than those of the control group. (E) Knockdown of ZFAS1 induced G1/S cell-cycle arrest. (F) The Transwell assay showed that the invasive numbers of the si-ZFAS1 groups were less than those of the control group. Three independent experiments were performed. Data are shown as the mean ± SD. ∗p < 0.05; ∗∗p < 0.01; ns, not significant.