Figure 5.

ZFAS1 contributes to CRC cell proliferation and lipogenesis by regulating SREBP1 expression

(A) The PABP2 protein levels in SW480 and RKO cells transfected with PABP2 siRNAs or negative control siRNA were examined by western blot. (B) The CCK-8 assay showed that knockdown of PABP2 inhibited the growth of SW480 and RKO cell lines. (C) The colony formation assay showed that the colony numbers of the si-PABP2 groups were less than those of the control group. (D) The SREBP1 protein levels in SW480 and RKO cells transfected with SREBP1 siRNAs or negative control siRNA were examined by western blot. (E) The CCK-8 assay showed that knockdown of SREBP1 inhibited the growth of SW480 and RKO cell lines. (F) The colony formation assay showed that the colony numbers of the si-SREBP1 groups were less than those of the control group. (G) The CCK-8 assay showed that rescue of SREBP1 partially reversed the effect of ZFAS1 downregulation on the proliferation of CRC cells. (H) The colony formation assay showed that rescue of SREBP1 partially reversed the effect of ZFAS1 downregulation on colony formation of CRC cells. (I) Cellular lipids were detected by relative kits, and the results showed that rescue of SREBP1 partially reversed the effect of ZFAS1 downregulation on lipogenesis in SW480 cells. Three independent experiments were performed. Data are shown as the mean ± SD. ∗∗p < 0.01.