Figure 6.

ZFAS1 promotes the malignant phenotype of CRC in vivo

(A) Whole tumor tissues collected from two groups of mice treated with ZFAS1 or negative control shRNA-transfected SW480 cells are shown. (B) The volumes of tumors from two groups of mice treated with ZFAS1 or negative control shRNA-transfected SW480 cells are shown. (C) The tumor weights from two groups of mice treated with ZFAS1 or negative control shRNA-transfected SW480 cells are shown. (D) Immunohistochemistry staining of human colorectal cancer tissues with SREBP1 and SCD1 antibodies is shown. (E) CRC cells transfected with ZFAS1 or negative control shRNA were injected into the PVS of 2-dpf wild-type zebrafish larvae. Images were taken using a stereomicroscope at 4 dpi. CM-DiI-positive areas in the yolk were quantified for proliferation, and CM-DiI-positive areas in the trunk were quantified for metastasis. The regions enclosed by the red dashed curve in the segmented images were selected for calculating tumor areas in the yolk or trunk. ∗∗p < 0.01.