Mutation of YS, F436, and IL, but not L48, inhibits the dendritic transport of α2B-AR in hippocampal neurons
(A) Positions of YS, L48, F436, and IL in α2B-AR.
(B) The dendritic expression of α2B-AR and its mutants. Hippocampal neurons were cultured and transfected with α2B-AR-GFP or its mutants together with dsRed vectors for 48 h. After fixation, receptor expression was visualized by confocal microscopy. Scale bar, 50 μm.
(C) Quantitative data shown in (B). Dendritic receptor expression was defined as the dendritic area expressing the receptor. The data are expressed as percentages of WT. Bars represent mean ± SE (n = 6–12 neurons in at least 3 individual experiments). One-way ANOVA test; ∗p < 0.001 versus WT.