Figure 6.

The LL motif of AT1R controls the ER export of VSVG

(A) A diagram showing the generation of the chimera VSVGct in which the C-terminal helical region of AT1R containing an LL motif (upper panel) was fused to the CT of VSVG (lower panel). TM, transmembrane domain.

(B) Subcellular distribution of VSVGct and its LL-AA mutant. HEK293 and SHSY5Y cells were transfected with GFP-tagged VSVGct or its LL-AA mutant. The cells were cultured at 40°C for 24 h (0 min) and then shifted to 32°C for 30 min. Scale bars, 10 μm.

(C) Quantitative data shown in (B). The data are expressed as percentages of the cells with VSVG expression at the Golgi with a total of at least 40 cells counted in each experiment. Bars represent mean ± SE (n = 3). Unpaired Student's t test; ∗p < 0.05 versus VSVGct.

(D) Diagrams showing the generation of VSVG and VSVGct mutants in which the DxE and/or LL motifs were mutated to alanines.

(E) Subcellular distribution of VSVG and VSVGct mutants. Scale bar, 10 μm.

(F) Quantitative data shown in (E). The data are expressed as percentages of the cells with VSVG expression at the Golgi with a total of at least 40 cells counted in each experiment. Bars represent mean ± SE (n = 3). Unpaired Student's t test; ∗p < 0.001 versus VSVG; ∗∗p < 0.001 versus VSVGct DxE-AxA.