Antibodies |
|
Mouse monoclonal anti-GM130 (clone 35) |
BD Biosciences |
610823 |
Rabbit polyclonal anti-giantin |
Abcam |
ab80864 |
Goat anti-rabbit IgG (H+L), Alexa Fluor 594 |
Thermo Fisher Scientific |
A-11012 |
Goat anti-mouse IgG (H+L), Alexa Fluor 594 |
Thermo Fisher Scientific |
A-11032 |
|
Chemicals, peptides, and recombinant proteins |
|
[3H]-RX821002 |
Perkin-Elmer |
NET1153250UC |
[3H]-CGP12177
|
Perkin-Elmer |
NET1061250UC |
[N-methyl-3H]-scopolamine methyl chloride |
Perkin-Elmer |
NET636250UC |
Lipofectamine 2000 |
Thermo Fisher Scientific |
11668019 |
Dulbecco's modified eagles medium |
HyClone |
SH30243.01HI |
Ham's F-12 nutrient mix |
Thermo Fisher Scientific |
11765054 |
Minimum essential medium |
Thermo Fisher Scientific |
11095080 |
Neurobasal-A medium |
Thermo Fisher Scientific |
12349015 |
B-27 supplement |
Thermo Fisher Scientific |
17504001 |
GlutaMax supplement |
Thermo Fisher Scientific |
35050061 |
Fetal bovine serum |
HyClone |
SH30396.03HI |
Penicillin-streptomycin solution |
HyClone |
SV30010 |
ProLong gold antifade mountant with DAPI |
Invitrogen |
P36931 |
|
Experimental models: Cell lines |
|
HEK293 |
ATCC |
CRL-1573 |
SHSY5Y |
ATCC |
CRL-2266 |
Sprague-Dawley rats |
Charles River Laboratories |
|
Primers (Table S1) |
This paper |
N/A |
|
Recombinant DNA |
|
α2B-AR-GFP |
(Wu et al., 2003) |
N/A |
α2B-AR YS-AA-GFP |
(Dong and Wu, 2006) |
N/A |
α2B-AR L48-A-GFP |
(Duvernay et al., 2009a) |
N/A |
α2B-AR F436-A-GFP |
(Duvernay et al., 2004) |
N/A |
α2B-AR IL-AA-GFP |
(Duvernay et al., 2004) |
N/A |
β2-AR-GFP |
(Wu et al., 2003) |
N/A |
β2-AR LL-AA-GFP |
(Duvernay et al., 2009a) |
N/A |
β2ARα2ct-GFP |
(Dong et al., 2010a) |
N/A |
β2ARα2ct IL-AA-GFP |
This paper |
N/A |
M3R-GFP |
(Wei et al., 2021) |
N/A |
M3R LL-AA-GFP |
This paper |
N/A |
DsRed |
Clontech Laboratories |
632466 |
VSVG-GFP |
Addgene |
11912 |
VSVG DxE-AxA-GFP |
This paper |
N/A |
VSVGct-GFP |
(Li et al., 2017) |
N/A |
VSVGct LL-AA-GFP |
This paper |
N/A |
VSVGct DxE-AxA-GFP |
This paper |
N/A |
VSVGct DxE-AxA/LL-AA-GFP |
This paper |
N/A |
α2A-AR-GFP |
(Li et al., 2017) |
N/A |
α2A-AR YS-AA-GFP |
(Dong and Wu, 2006) |
N/A |
DsRed2-ER |
(Dong and Wu, 2006) |
N/A |
|
Software and algorithms |
|
ImageJ |
NIH |
imagej.nih.gov/ij/ |