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. 2022 Jan 5;21:6. doi: 10.1186/s12934-021-01728-7

Table 1.

Acceptor proteins quantities produced in the same E. coli SDB1 background but with the pgl locus in plasmid (SDB1 pACYCpgl) or inserted in the chromosome of SDB1 (SDB1pgl) at 30 °C and 37 °C (X indicates ORF inserted coding for G-SodB/G-FlpA/G-NetB/G-ExoA)

Temperature (°C) E. coli background Acceptor proteins
G-SodB G-FlpA G-NetB G-ExoA
30 SDB1 pACYCpgl pEXT20_X 0.4 0.92 0.40 1.79
SDB1pgl pEXT20_X 0.24 0.90 0.28 0.83
37 SDB1 pACYCpgl pEXT20_X 0.54 0.41 0.28 0.71
SDB1pgl pEXT20_X 0.45 1.09 0.28 0.55