Figure 4.

Anti-EFG1 2′OMe oligomer effect on C. albicans cell morphology and progression into the fat body of G. mellonella

(A) Histological images of larvae infected with C. albicans (at 24 and 48 h) and treated with a single dose (0 h post infection) and with a double dose (0 and 12 h post infection) of 40 and 100 nM of anti-EFG1 2′OMe oligomer. The larvae sections were labeled with periodic acid Schiff coloration. The magnification images were at 400×. (B) Levels of EFG1 gene expression of larvae treated with a double dose of 100 nM of anti-EFG1 2′OMe oligomer evaluated by quantitative real-time PCR and analyzed by the ΔCt method and normalized to the CaACT1 mRNA levels after 4 and 24 h post infection. Control represents the infected larvae treated only with PBS after 12 h post infection. Error bars represent standard deviation. ∗∗∗Significant difference among control and a double dose of 100 nM of anti-EFG1 2′OMe oligomer at 24 h post infection (p < 0.001).