Figure 1. Interaction with the EJC is required for Btz function in the ovary.

• A
  Diagram of the genomic rescue constructs in which wild‐type Btz (Btz‐WT) or Btz with the H215A and D216A mutations (Btz‐HD) is C‐terminally tagged with GFP and driven by its endogenous regulatory sequences.
• B
  Co‐immunoprecipitation of HA‐tagged Btz‐WT or Btz‐HD with Myc‐tagged GFP or eIF4AIII from transfected S2R+ cells using Myc‐Trap Magnetic Agarose beads. The upper Western blot is blotted with anti‐Myc and the lower with anti‐HA. Input samples are shown on the left and immunoprecipitates on the right. Btz‐WT co‐immunoprecipitates with eIF4AIII, but Btz‐HD does not, although the protein is equally stable. The asterisk represents a likely cleavage product of Btz.
• C–F
  Stage 10 egg chambers stained with anti‐Vasa (C‐F) or anti‐GFP (E’, F’). (C) wild‐type; (D) germline clones homozygous for the null allele btz² ; (E) btz² germline clones rescued with Btz‐WT; (F) btz² germline clones rescued with Btz‐HD. Posterior is to the right. Scale bars, 80 μm. Vasa localization to the posterior pole of the oocyte is lost in btz mutants and is rescued by the wild‐type but not the EJC interaction‐defective transgene. Wild‐type Btz‐GFP, but not the mutant form, is also slightly enriched at the posterior pole.

Source data are available online for this figure.