Figure EV3. Btz regulates P‐Mad levels at the NMJ independently of the EJC.

• A–F
  Confocal images of the NMJ on larval muscles 6 and 7 in segment A3 (A‐D) or the larval ventral nerve cord (E, F), stained with anti‐P‐Mad. (A, E) btz² /+ control; (B, F) btz² /Df(3R)BSC497; (C) Btz‐WT; btz² /Df(3R)BSC497; (D) Btz‐HD; btz² /Df(3R)BSC497. Scale bars, 20 μm. P‐Mad is lost from the synapse but not from neuronal cell bodies in btz mutants, and is rescued by both the wild type and EJC interaction‐defective btz transgenes.