FIG. 6.

Expression of gut morphogenetic factors in growth-impaired MTG8 exon 2-targeted mice. S1 protection analysis of RNA from proximal (je) and distal (co) gut segments from a P7 MTG8 exon 2-targeted homozygous pup (weight, 2.78 g) and a control (wt) littermate (weight, 5.98 g) was carried out. tRNA, negative control for probe hybridization. The probes are described in Table 1. The top band corresponds to residual undigested full-length probe. Filled arrows indicate the expected protected fragment for each probe. For reference purposes, protection by an actin probe added to the same hybridization mixture is shown at the bottom (white arrow). The numbers on the left indicate the molecular size markers in nucleotides. The apparent minor differences with some probes were inconsistent.