Representative images from the U2OS‐FokI data summarized in Fig
3A. U2OS‐LacR‐FokI cells were transfected with the indicated siRNA. The following day, DSB induction was performed with 4‐OHT and Shield‐1. Cells were processed for immunofluorescence microscopy using primary antibodies directed against 53BP1, RIF1, BRCA1 and RAD51. Scale bar = 2.5 µm.