Table 1.
Antibodies used for immunoblotting, immunofluorescence, flow cytometry.
| Antibody | Clone/ID | Company |
|---|---|---|
| Flag | M2 | Sigma‐Aldrich |
| POGZ | ab171934 | Abcam |
| HP1‐gamma | ab213167 | Abcam |
| HP1‐alpha | ab109028 | Abcam |
| HP1‐beta | MAC353 | Abcam |
| gamma‐H2AX | JBW301 | EMD‐Millpore |
| BARD1 | ab226854 | Abcam |
| BRCA1 | 07‐434 | EMD‐Millpore |
| RAD51 | 70‐001 | Bio‐Academia |
| 53BP1 | H‐300 | Santa Cruz |
| alpha‐Tubulin | DM1A/ab7291 | Abcam |
| pATR (S1989) | D5K8W | C.S.T. |
| pATM (S1981) | D25E5 | C.S.T. |
| pCHK1 (S345) | 133D3 | C.S.T. |
| pCHK2 (T68) | C13C1 | C.S.T. |
| pKAP1 (S824) | ab70369 | Abcam |
| ATR | E1S3S | C.S.T. |
| ATM | ab32420 | Abcam |
| CHK1 | 2G1D5 | C.S.T. |
| CHK2 | ab109413 | Abcam |
| KAP1 | ab10484 | Abcam |
| gamma‐H2AX | ab26350 | Abcam |
| Rat Anti mouse IgG1 PE | 553445 | BD Biosciences |
| Biotin Rat anti‐mouse IgM | 553406 | BD Biosciences |
| Biotin Rat anti‐mouse IgG1 | 553441 | BD Biosciences |
| Biotin Rat anti‐mouse IgG3 | 553404 | BD Biosciences |
| Biotin Rat anti‐mouse IgG2b | 553393 | BD Biosciences |
| Biotin Rat anti mouse IgG2a | 553446 | BD Biosciences |
| Biotin Rat anti‐mouse IgG2a | 553388 | BD Biosciences |
| PE anti‐IgG1 | A85‐1 | BD Biosciences |
| Rabbit Anti‐Mouse HRP conjugate | AP160P | Sigma‐Aldrich |
| Goat Anti‐Rabbit HRP conjugate | 12‐348 | Sigma‐Aldrich |
| HA | A190‐ 108A | Bethyl |
| goat anti‐mouse Alexa 488 | 4408S | C.S.T. |
| goat anti‐rabbit Alexa 647 | 4414S | C.S.T. |
| Anti‐Rat Goat Alexa Fluor 488 | A‐11070 | Thermofisher |
| Anti‐Mouse Goat Alexa Fluor 647 | A‐21237 | Thermofisher |
| mCherry | 1C51 | Abcam |
| GAPDH | 1D4 | Novus Biologicals |