Table 2.
Description of the primers used in this study.
| A): List of primers used for quantitative RT‐PCR | |||
|---|---|---|---|
| Species | Target | Forward sequence | Reverse sequence |
| Human | POGZ | ACCCAGTTTGTTAAGCCGACA | ACATCGTGGACATATTTTCCGTC |
| Human | GAPDH | AATCCCATCACCATCTTCCA | TGAGTCCTTCCACGATACCA |
| Mouse | AID | GTGCCACCTCCTGCTCACTGG | TTCATGTAGCCCTTCCCAGGC |
| Human | BRCA1‐1 | GAAACCGTGCCAAAAGACTTC | CCAAGGTTAGAGAGTTGGACAC |
| Human | BRCA1‐2 | ACCTTGGAACTGTGAGAACTCT | TCTTGATCTCCCACACTGCAATA |
| Human | RAD51‐1 | CAACCCATTTCACGGTTAGAGC | TTCTTTGGCGCATAGGCAACA |
| Human | RAD51‐2 | CGAGCGTTCAACACAGACCA | GTGGCACTGTCTACAATAAGCA |
| Human | PALB2‐1 | AGGATCTCTCACCGCAGCTAA | TCAGGCCCAACATCAAGTGTG |
| Human | PALB2‐2 | ATCCTGCTAGATCACCAGTAACT | TCAAGGTGCTGACTACTACCG |
| Human | BRCA2‐1 | CACCCACCCTTAGTTCTACTGT | CCAATGTGGTCTTTGCAGCTAT |
| Human | BRCA2‐2 | TGCCTGAAAACCAGATGACTATC | AGGCCAGCAAACTTCCGTTTA |
| Human | BARD1‐1 | CTGCTCGCGTTGTACTAACAT | TCCAATGCAGTCACTTACACAAT |
| Human | BARD1‐2 | GGTATCCTTCTGTAGCCAACCA | GGAGCCACTTGCTAGTAAGTCT |
| B): Sequence of the sgRNAs used to target murine and human POGZ by CRISPR/Cas9 technology | ||
|---|---|---|
| Species | Target | sgRNA sequence |
| Mouse | Pogz | TACAGTCCCGGCTACCCAGT |
| Mouse | Pogz | TATTAGCTCATCCTAGTTCC |
| Mouse | Pogz | TACCCAGATAATAGTGATAG |
| Mouse | Pogz | GTTGTCCGTGAAATAGTAGG |
| Human | POGZ | CACCGTGCCCCGCTGCTACTAACGG |
| Human | POGZ | AAACCCGTTAGTAGCAGCGGGGCAC |
| C): Primers used for the generation of a siRNA‐resistant POGZ construct and the different deletion mutants of POGZ | |||
|---|---|---|---|
| Target | Description of the construct | Forward sequence | Reverse sequence |
| POGZ | siRNA‐resistant POGZ (C942T, A948G, C954A) | GCTTGGGATTCGTCGTCTGATTTGATTGGCCTGGAGACTGAACA | TGTTCAGTCTCCAGGCCAATCAAATCAGACGACGAATCCCAAGC |
| POGZ | N‐800 | CCACGGAAGAGCCCCCCTGAAAGTCTTCAC | GTGAAGACTTTCAGGGGGGCTCTTCCGTGG |
| POGZ | N‐848 | GGGGACAAGTTTGTACAAAAAAGCAGGCTTCATGGCGGACACCGACCT | GGGGACCACTTTGTACAAGAAAGCTGGGTTTCACAGGATGCTGCTGGATC |
| POGZ | 800‐C | CCACGGAAGAGCCCCCCTGAAAGTCTTCAC | GTGAAGACTTTCAGGGGGGCTCTTCCGTGG |
| POGZ | 1096‐C | GTGAAGACTTTCAGGAGGTAGGGTGTGGGCCA | TGGCCCACACCCTACCTCCTGAAAGTCTTCAC |
| POGZ | 366‐848 | GGGGACAAGTTTGTACAAAAAAGCAGGCTTCATGGTATTTGACCTCCAGGATGG | GGGGACCACTTTGTACAAGAAAGCTGGGTTTCACAGGATGCTGCTGGATC |
| POGZ | ΔHP1 | CGGAAGAGCCCCCCACGGGGACTC | GAGTCCCCGTGGGGGGCTCTTCCG |
| POGZ | 848‐C | GAGGAGGAGGAGTTGGAGCCACCCAAGTATTT | AAATACTTGGGTGGCTCCAACTCCTCCTCCTC |