Table 2.

Transmission efficiency of the cycb1;2 and cycb1;3 mutant alleles in reciprocal crosses of a quadruple mutant with wild type.

Parental genotypes (female × male)	Mutant allele		Number of seeds (n)
	cycb1;2	cycb1;3
cycb1;1 −/− cycb1;2+/− cycb1;3 +/− cycb1;4 −/− × Col‐0	0%****	92.7%****	192
Col‐0 × cycb1;1 −/− cycb1;2 +/− cycb1;3 +/− cycb1;4 −/−	70.84%****	30.2%****	192

A transmission efficiency of 100% indicates full transmission of the mutant allele, that is, 50% of the genotyped F1 seedlings are heterozygous. A transmission efficiency may be higher than 100% if one of the two alleles is transmitted in a reduced manner, conversely making the other allele overrepresented. A z‐test for one proportion was performed to test if the observed transmission frequencies differ from expected values and the significance level was corrected for multiple comparisons using Bonferroni. Asterisks indicate significant differences from expected values (****P < 0.0001). Primers used for genotyping are listed in Table EV1.