Figure EV2. Hypoxia itself causes the induction of GR‐responsive genes.

• A, B
  Female C57BL/6J mice were put in normoxia or hypoxia for 6 h (A) or 24 h (B), injected with PBS or DEX (10 mg/kg) and 2 h later, liver was isolated for analyses. N = 3 per group for a single RNA‐seq. (A) Heatmap representing stress GRE genes induced by hypoxia (6 h) and DEX in normoxia. Log₂ values are shown of the counts of the stress GRE genes based on the RNA‐seq data (LFC > 1 and P ≤ 0.05). (B) Heatmap representing stress GRE genes induced by hypoxia (24 h) and DEX in normoxia. Log₂ values are shown of the counts of the stress GRE genes based on the RNA‐seq data (LFC > 1 and P ≤ 0.05).
• C, D
  Females C57BL/6J and ADX mice were put in normoxia (N) and hypoxia (H, 6 and 24 h) and the expression of HIF target genes were evaluated in the liver via RT–qPCR. (C) Fold inductions (H/N) of HIF target genes in C57BL/6J and ADX mice. P‐value was calculated using a two‐way unpaired Student’s t‐test. (D) Examples of the expression levels of HIF target genes. N = 3 per group.

Data information: All bars represent mean ± SEM. P‐values were calculated using two‐way ANOVA followed by post‐hoc Šídák’s multiple comparisons test to correct for multiple testing during the pairwise multiple comparisons, except if otherwise stated. ***P < 0.001, **P < 0.01, *P ≤ 0.05.