Skip to main content
. Author manuscript; available in PMC: 2022 Jan 5.
Published in final edited form as: Cell Rep. 2021 Nov 30;37(9):109933. doi: 10.1016/j.celrep.2021.109933

Figure 2. DAergic inhibition of pyramidal cells in ACC is exclusively mediated by D1 receptors.

Figure 2.

(A) Typical current-clamp recordings illustrating alterations in pyramidal firing activity following application of 10 μM SKF81297, 5 μM SCH23390 + 50 μM DA, and 10 μM quinpirole.

(B) Top panel: quantitative group analysis of cell excitability; SKF81297 induces a significant decrease in excitability, whereas 5 μM SCH23390 + 50 μM DA and 10 μM quinpirole did not have any inhibitory effect. Bottom panel: input-output plots recorded during each of 4 different treatments (n = 6–10) are shown.

(C) Left panel: typical traces illustrating the impact of various DAergic ligands on input resistance. Right panel: 10 μM SKF81297 induces a significant decrease in input resistance, whereas 5 μM SCH23390 + 50 μM DA and 10 μM quinpirole did not (n = 6–10).

(D) Left panels: electrophysiological current-clamp traces illustrating 10 μM DHPG-induced persistent firing along with the reversible inhibitory effects of 50 μM DA and 10 μM SKF81297. Right panel: quantitative group analysis illustrating the significant decrease in firing frequency during persistent firing following application of 50 μM DA or 10 μM SKF81297 in comparison to the control group (DHPG alone; n = 11–13) is shown.

Values represented as means ± SEM: *p < 0.05; ***p < 0.001; ****p < 0.0001.