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. Author manuscript; available in PMC: 2022 Jan 5.
Published in final edited form as: Cell Rep. 2021 Nov 30;37(9):109933. doi: 10.1016/j.celrep.2021.109933

Figure 3. DAergic inhibition depends on HCN channels.

Figure 3.

(A) Treatment with the HCN channel blocker 10 μM ZD7288 occludes the inhibitory effects of 50 μM DA. Representative current-clamp traces showing the effects of 10 μM ZD7288 on excitability (left) and group analysis (right; n = 8) are shown.

(B) 10 μM ZD7288 blocks the 50 μM DA-induced decrease in input resistance. Representative current-clamp traces showing the effects of 10 μM ZD7288 on input resistance (left) and quantitative group analysis (right; n = 7) are shown.

(C) Quantitative effects of 10 μM ZD7288 on firing frequency (left) and input resistance (right) at baseline (n = 8).

(D) Both 50 μM DA and 10 μM SKF81297 induce a significant increase in resting membrane potentials of layer 2/3 pyramidal neurons of the ACC. This effect is lost in presence of the D1R antagonist SCH23390 (5 μM) or when HCN channels are blocked with ZD7288 (10 μM; n = 7–17).

Values represented as means ± SEM: *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.