Table 2.

Effects of 5-aza-2′-deoxycytidine/trichostatin A on the numbers of CD34⁺CD90⁺ cells

Culture condition	Total nucleated cells (×104)	(%) CD34+CD90+ cells	Fold expansion of CD34+CD90+ cellsa	(%) S/G2/M
SFT + IL-3/SFT +5azaD/TSA (16 h 5azaD/48 h TSA)	42.7 ± 9.7	31.4 ± 4.1	10.9 ± 0.8*,†	12.5 ± 3.8‡
SFT/SFT +5azaD/TSA (16 h 5azaD/48 h TSA)	7.0 ± 1.3	42.9 ± 3.9	2.0 ± 0.3*	6.6 ± 2.9‡
SFT + IL-3/SFT +TSA/5azaD (16 h TSA/48 h 5azaD)	8.3 ± 0.5	4.6 ± 0.6	0.3 ± 0.1†	ND

The 5 × 10⁴ CD34⁺ cells/well were cultured using the protocol described below.

SFT/SFT indicates that SCF + FL + TPO were added to culture for the first 48 hours that lacked interleukin-3 (IL-3), and stem cell factor (SCF)+Flt3 ligand (FL)+thrombopoietin (TPO) were added to culture for the final 7 days of the 9-day culture period.

SFT + IL-3/SFT indicates culturing in SCF + FL + TPO + IL-3 for the first 48 hours, followed by SCF + FL + TPO for the terminal 7 days; 16 hours 5-aza-2′-deoxycytidine (5azaD)/48 hours TSA indicates that 5azaD was added to the culture at 16 hours and trichostatin A (TSA) was added at 48 hours; 16-hour TSA/48-hour 5azaD indicates that TSA was added to culture at 16 hours and 5azaD was added at 48 hours.

Each value represents the mean of three independent experiments ± standard error.

^aFold expansion was calculated as the total numbers of CD34⁺CD90⁺ cells at the end of culture period (output cells) over the number of primary CD34⁺CD90⁺ cell numbers (input number of cells started at the beginning of the culture).

ND = not determined.

^*p < 0.01;

^†p < 0.01;

^‡p < 0.05.