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. Author manuscript; available in PMC: 2022 Jan 5.
Published in final edited form as: Exp Hematol. 2009 Jul 2;37(10):1131–1142. doi: 10.1016/j.exphem.2009.06.007

Figure 4.

Figure 4.

(A) results of bisulfite sequence analysis of purified erythroid cells of days 7, 11, and 14 cultured erythroid progenitor cells (EPC) and 3 representative samples of purified erythroid cells of bone marrow (BM) aspirates of normal and bled baboons. Each row represents the sequence analysis of a single pCR4-derived clone containing the polymerase chain reaction (PCR) amplicon of the γ-globin promoter region following bisulfite modification. Columns show the level of DNA methylation at the respective −54, −51, +5, +16, and +48 CpG sites within the 5′ region of the γ-globin gene. Methylated CpG (red); unmethylated CpG (green); baboon-specific polymorphic sites resulting in the absence of the CpG dinucleotide (yellow). (B) Analysis of the proportion of DNA methylation at each respective CpG site (−54, −51, +5, +16, +48) in purified erythroid cells of day 7, 11, 14 EPC, and normal and bled bone marrow (BM). (C) Analysis of the number of methyl CpG present in sequenced clones of amplified, bisulfite-treated DNA purified from purified erythroid cells from day 7, day 11, day 14 EPC, and normal and bled BM.