FIG. 2.

Western blot and RT-PCR analysis of the expression of the various constructs. The upper panels show expression of the various MLL fusion protein constructs in transiently transfected BOSC cells using antibodies to MLL, ELL, or EAF1. The lower panels show RT-PCR expression analysis in the transduced myeloid clonogenic cells harvested from primary methylcellulose cultures. Amplification from reverse transcribed cDNA is indicated by a plus symbol (+). To exclude amplification of integrated retroviral genomic DNA, a no-RT control is indicated by a minus symbol (−).