Table 2.
List of antibodies used. Details of antibodies used in western blot, immunofluorescent staining, ChIP assay, and immuno‐histochemistry
| Target | Concentration | Catalog number | Manufacturer | Application |
|---|---|---|---|---|
| α‐SMA | 1:1000 | m0851 | Dako | Western blotting |
| GAPDH | 1:10 000 | mab374 | Millipore | |
| VEGF | 1:500 | sc152 | Santa Cruz | |
| F4/80 | 1:100 |
Allophycocyanin (APC): 17‐4801‐82 Phycoerythrin (PE): 12‐4801‐82 Fluorescein isothiocyanate (FITC): 11‐4801‐85 |
eBioscience | Immunofluorescent staining |
| α‐SMA | 1:300 | F3777 | Sigma | |
| CD31 | 1:100 | 102514 | Biolegend | |
| hCD68 | 1:100 | F7135 | Dako | |
| mCd68 | 1:100 | 137012 | Biolegend | |
| CD206 | 1:300 | AF488: 141710 APC: 141708 | Biolegend | |
| p‐Smad3 | 1:200 | 600‐401‐919 | Rockland | |
| bFGF | 1:100 | sc74412 | Santa Cruz | |
| Anti‐GFP | 1:20 | 338008 | Biolegend | |
| Col‐1 | 1:100 | 1310‐01 | SouthernBiotech | |
| p‐Smad3 | 1:100 | sc‐517575 | Santa Cruz | IHC |
| α‐SMA | 1:100 | M0851 | DAKO | |
| CD68 | 1:100 | M0814 | DAKO | |
| Smad3 | 1:50 | 9523S | Cell Signaling Technology | ChIP |