Figure 1.

Heterogeneous T-cell infiltration and HLA class I antigen expression in different metastases from patient Ma-Mel-48. A, localization and excision date of three sequential metastases from patient Ma-Mel-48. B, serial sections from cryopreserved Ma-Mel-48a and −48c metastatic lesions were analyzed for the expression of HMB-45 (melanoma cells), CD3 (T cells), and HLA class I antigens by IHC. Red staining indicates positive cells. C, flow cytometric analysis of total HLA class I antigen expression on melanoma cell lines established from the different metastases of patient Ma-Mel-48. Cells were stained with mAb W6/32. Histograms from one representative of three independent experiments are shown. Numbers indicate mean fluorescence intensity (MFI).