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. Author manuscript; available in PMC: 2022 Mar 14.
Published in final edited form as: Nat Aging. 2021 Sep 14;1(9):850–863. doi: 10.1038/s43587-021-00110-x

Figure 4. Single-cell profiling of CD14+ monocytes from PD and control subjects.

Figure 4.

(A) Generation of scRNA-seq from seven PD and three controls yielded 19,144 cells. Uniform Manifold Approximation and Projection (UMAP) visualization representing the six clusters including CD14++/CD16− classical monocytes (purple) CD14++/CD16+ intermediate cluster (green). (B) Comparison of the relative levels of expression of mitochondrial and proteasomal genes in the classical vs. intermediate monocytes using normalized effect (without considering diagnosis) 34,60. n=10 independent samples (C) Volcano plot showing the normalized effect within CD14++/CD16+ intermediate cluster of PD-monocytes and controls (x-axis) and their significance (y-axis, −log10 P-value). DEGs at q-value < 0.05 are highlighted in red (upregulated genes) and in blue (downregulated genes). Wald statistic is used for statistical test.