Skip to main content
. Author manuscript; available in PMC: 2022 Jan 5.
Published in final edited form as: Cytometry A. 2014 Sep 2;85(11):953–961. doi: 10.1002/cyto.a.22563

Figure 3.

Figure 3.

Inhibition by NU1025 of PARP-1 activation induced by TPT or RT in human GBM cell lines. D54 and U251 cells were treated with the indicated doses of topotecan (TPT), ionizing radiations (RT) and NU1025 (10 μM). PARP-1 activity was determined by immunofluorescent staining of poly-ADP-ribosylation (PAR) of the DNA following a 4 h incubation. PI staining was used as an indicator of nuclear compartment. Representative staining of PAR and PI staining of D54 and U251 cells treated with TPT, RT, and NU1025 are shown.