FIG. 3.

Analysis of mouse Brainiac 1 expression in the CNS by in situ hybridization. Throughout the panel, standard phase-contrast micrographs are on the left; dark-field micrographs show the in situ hybridization signal on the right. (A and B) Expression of mouse Brainiac 1 in the ventricles of the developing CNS at day 12.5 of gestation. Expression in the mantle zone appears to be higher than expression in the ventricular zone (B, arrow). (C to F) Mouse Brainiac 1 is expressed in the ganglion cell layer (F, arrow) of the developing retina at postnatal day 1. (G to H) Mouse Brainiac 1 is expressed in the dentate gyrus and the CA regions of the hippocampus at postnatal day 10. An identical expression pattern was observed in the hippocampus at postnatal day 1. Control experiments with sense probes were negative (data not shown).