Table 2.
Summary of the RNA-seq data
| Sample | Raw reads | Clean reads | Clean bases (Gb) | Q20 (%) | Q30 (%) | GC (%) | Total reads | Total map | Unique map |
|---|---|---|---|---|---|---|---|---|---|
| SUS_A | 48,504,690 | 48,490,522 | 7.27 | 95.86 | 90.03 | 40.07 | 48,490,522 | 41,725,916 (86.05%) | 41,106,490 (84.77%) |
| SUS_B | 50,196,366 | 50,181,356 | 7.53 | 96.22 | 90.68 | 41 | 50,181,356 | 43,847,577 (87.38%) | 43,175,889 (86.04%) |
| SUS_C | 53,504,154 | 53,487,908 | 8.02 | 96.15 | 90.6 | 40.3 | 53,487,908 | 46,166,534 (86.31%) | 45,443,847 (84.96%) |
| IMI_A | 42,456,440 | 42,443,694 | 6.37 | 96.3 | 90.88 | 40.97 | 42,443,694 | 37,056,407 (87.31%) | 36,520,464 (86.04%) |
| IMI_B | 63,185,144 | 63,166,110 | 9.47 | 96.14 | 90.42 | 43.41 | 63,166,110 | 56,409,149 (89.3%) | 55,417,093 (87.73%) |
| IMI_C | 47,763,844 | 47,749,700 | 7.16 | 96.2 | 90.74 | 40.23 | 47,749,700 | 41,246,966 (86.38%) | 40,629,937 (85.09%) |
| ACE_A | 55,560,384 | 55,543,710 | 8.33 | 96.15 | 90.59 | 40.52 | 55,543,710 | 47,371,626 (85.29%) | 46,617,991 (83.93%) |
| ACE_B | 48,844,738 | 48,830,222 | 7.32 | 96.12 | 90.51 | 40.18 | 48,830,222 | 41,837,768 (85.68%) | 41,197,173 (84.37%) |
| ACE_C | 44,189,186 | 44,175,744 | 6.63 | 96.03 | 90.38 | 40.18 | 44,175,744 | 37,349,722 (84.55%) | 36,757,531 (83.21%) |
| THI_A | 48,398,214 | 48,383,968 | 7.26 | 95.81 | 89.64 | 42.38 | 48,383,968 | 41,554,580 (85.89%) | 40,870,267 (84.47%) |
| THI_B | 53,127,326 | 53,111,186 | 7.97 | 96.08 | 90.53 | 40.09 | 53,111,186 | 44,531,359 (83.85%) | 43,839,726 (82.54%) |
| THI_C | 50,190,878 | 50,176,074 | 7.53 | 96.16 | 90.59 | 41.02 | 50,176,074 | 41,993,835 (83.69%) | 41,330,531 (82.37%) |
SUS_A, B, and C: three repeats from susceptible MEAM1 cryptic species raised under control conditions without contact of any pesticide; IMI_A, B and C: three repeats from susceptible MEAM1 cryptic species selected continuously by imidaclorprid for 10 generations; ACE_A, B and C: three repeats from susceptible MEAM1 cryptic species selected continuously by acetamiprid for 10 generations; THI_A, B and C: three repeats from susceptible MEAM1 cryptic species selected continuously by thiamethoxam for 10 generations; Q20: the percentage of bases with a Phred value > 20; Q30: the percentage of bases with a Phred value > 30. Total reads: Number of clean reads after quality control; Total map: Number and percentage of clean reads mapped with reference genome; Unique map: Number and percentage of clean reads mapped with unique locus of reference genome