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. 2022 Jan 4;19:3. doi: 10.1186/s12974-021-02367-w

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Genetic (Cx3cr1-iDTR) and pharmacological (PLX5622) model of microglia ablation leads to efficient microglia ablation. A Generation of genetic microglia ablation model in Cx3cr1-iDTR mice. B Experimental timeline for genetic CNS specific microglia ablation. C Quantification of microglial cell numbers in control (Cx3cr1-Cre n = 7 or iDTR n = 3) or microglia ablation mice (Cx3cr1-iDTR n = 7, p < 0.001, One-way ANOVA). D representative images of microglia cell (IBA1-immunostaining) in control and ablated mice. F Experimental timeline for PLX5622 mediated microglia ablation. G, H representative images of microglia cell (IBA1-immunostaining) in control and PLX5622-ablated mice. I Quantification of microglial cell numbers in control (control diet for 7 days, n = 3) or mice that were subjected to PLX5622 for different days (n = 3 for each time points, Mean ± SEM, ** or *** indicates < 0.01 or p < 0.001 compared to control, one-way ANOVA). Quantification result is from one cohort of mice with similar results observed from > 2 other independent experiments