Fig. 3.

Pharmacological microglia ablation (PLX5622) does not lead to changes in CSF/ventricular spaces in vivo. A Experimental timeline for PLX5622 mediated microglia ablation and MRI quantification. B T2-Weighted MRI shows no significant difference in control diet or PLX5622 diet mice before or after the diet treatment. C MRI image-based quantification of the ventricular volume in control and PLX5622 microglia ablated mice using serial coronal sections of T2-weighted MRI before and after 7 days of control of PLX5622 treatment. (n = 3 for each group, Mean ± SEM, and no statistical differences among any of the groups by two-way ANOVA with Tukey post-hoc analysis). Quantification result is from one cohort of mice with similar results observed from > 2 other independent experiments