Table 1.
Primer/Probe used for qRT-PCR
| Primer/probe set | ||
|---|---|---|
| Hmbs | Forward Primer | 5'-TCC CTG AAG GAT GTG CCT AC-3' |
| Reverse Primer | 5'-ACA AGG GTT TTC CCG TTT G-3' | |
| Probe | Universal Probe Library: Probe 79—Roche | |
| Hprt1 | Forward Primer | 5'-TGA TAG ATC CAT TCC TAT GAC TGT AGA-3' |
| Reverse Primer | 5'- AAG ACA TTC TTT CCA GTT AAA GTT GAG-3' | |
| Probe | Universal Probe Library: Probe 22—Roche | |
| Iba1 | Forward Primer | 5'-GGATTTGCAGGGAGGAAAA -3' |
| Reverse Primer | 5'- TGGGATCATCGAGGAATTG-3' | |
| Probe | Universal Probe Library: Probe 3—Roche | |
| Gfap | Forward Primer | 5'- TGGAGGAGGAGATCCAGTTC-3' |
| Reverse Primer | 5'- AGCTGCTCCCGGAGTTCT-3' | |
| Probe | Universal Probe Library: Probe 69—Roche | |
| TNFα | Forward Primer | 5'- CTGTAGCCCACGTCGTAGC-3' |
| Reverse Primer | 5'- TTGAGATCCATGCCGTTG-3' | |
| Probe | Universal Probe Library: Probe 25—Roche | |
| IL-1β | Forward Primer | 5'- AGTTGACGGACCCCAAAAG-3' |
| Reverse Primer | 5'- AGCTGGATGCTCTCATCAGG-3' | |
| Probe | Universal Probe Library: Probe 38—Roche | |
| IL6 | Forward Primer | 5'- GCTACCAAACTGGATATAATCAGGA-3' |
| Reverse Primer | 5'- CCAGGTAGCTATGGTACTCCAGAA-3' | |
| Probe | Universal Probe Library: Probe 6—Roche | |
| KC/GRO | Forward Primer | 5'- ACTCCAACACAGCACCATGA-3' |
| Reverse Primer | 5'- TGGTCTGCAGGCACTGAC-3' | |
| Probe | Universal Probe Library: Probe 49—Roche |