Fig. 5.

RotaRod performance (A–C) and acoustic startle response (D–F) where recorded on PND 20 for male (n = 8) and female (n = 8) pups dosed between PND 2–5 (A, D), PND 7–10 (B, E), and PND 17–20 (C, F). Female pups dosed with TiO₂ NP between PND 7–10 showed significantly decreased performance in the RotaRod test, while female pups dosed between PND 2–5 showed decreased response in the acoustic startle response test. Box and whisker plots where, the box extends from the 25th to 75th percentile and shows the median value, while the whiskers show the minimum and maximum value. TiO₂ NP dose groups are shown in black and vehicle control in gray. Statistical analyses were done using Mann–Whitney U test: *P-value < 0.05, **P-value < 0.01, ***P-value < 0.005