Fig. 2.

Hsa_circ_0003258 promotes the aggressiveness of PCa cells. A DU145 cells transfected with two siRNAs specifically targeting hsa_circ_0003258(S1, S2) and hsa_circ_0003258 overexpression plasmid for 48 h, and the level of hsa_circ_0003258 was detected by qRT-PCR. B-C Wound healing assay detected the migratory capacity of PCa cells after changing the level of hsa_circ_0003258. Scale bar, 100um. D-E Transwell assay detected the migratory capacities of PCa cells after changing the level of hsa_circ_0003258. Scale bar, 100um. F C4–2 cells transfected with two siRNAs specifically targeting hsa_circ_0003258(S1, S2) and PC3 cells transfected with hsa_circ_0003258 overexpression plasmid for 48 h, and the level of hsa_circ_0003258 detected by qRT-PCR. G-H Wound healing assay detected the migratory capacity of PCa cells after changing the level of hsa_circ_0003258. Scale bar, 100um. I-J Transwell assay detected the migratory capacity of PCa cells after changing the level of hsa_circ_0003258. Scale bar, 100um. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001