TABLE 2.
Relevant genotypea | Growth withb:
|
||
---|---|---|---|
Vector | ACC1-794c | SNF4-204d | |
snf1Δ10 | − | + | − |
snf4Δ1 | − | + | + |
spt15-328 | − | −/+ | + |
spt15-341 | − | −−/+ | + |
ino2Δ | − | − | − |
ino4Δ | − | − | − |
The following yeast strains were tested: PY133, FY454, KY214, KY231, PY188, and PY190.
Symbols indicate relative growth on solid media lacking inositol after 3 days at 30°C. Strong, weak, very weak, and no growth are indicated by +, −/+, −−/+, and −, respectively.
pPS65 contains ACC1-794 sequences (see Materials and Methods).
pPS47 contains SNF4-204 sequences (67).