Summary of XFS, MST and TSA results for nsp10 targeting fragment hits and calculated properties of the fragments. The ΔT_m values of nsp10 in the presence of fragments obtained from nanoDSF and fragment affinities for nsp10 as determined by MST are given. Molecular weight, MW; polar surface areas, PSA; calculated logP, clogP; HBA, hydrogen bond acceptor; HBD, hydrogen bond donor; MolLogS, calculated solubility; lifc, ligand-induced fluorescence change. TSA experiments were conducted in duplicate, whereas MST experiments were carried out in triplicate and are therefore presented individually or as average ± SD. The average T_m of nsp10 without any ligand under the same assay conditions was determined to be 46.6 ± 0.4 °C (n = 11).

Fragment ID	Binding site	MST nsp10 Kd [mM]	TSA nsp10 ΔTm [°C]	MW [Da]	clogP	HBA and HBD	tPSA [Å2]	MolLogS [Log (moles L−1)]
VT00022	1 & 2	>20 mM	Atypical curve	144.07	1.35	1/1	24.39	−1.60
VT00221	2	7.4 ± 3.1	−2.6 and −2.3	144.18	0.80	1/2	38.38	−2.21
VT00239	2	1.9 ± 0.7	−3.6 and −3.5	137.19	−0.42	1/2	41.62	−1.05
VT00265	2	lifc	0 and −1.3	133.15	−0.38	1/3	50.41	−1.10