TABLE 2.
Growth of S. cerevisiae at pH 6.1 on different nitrogen sources
| Nitrogen sourcea | Concentration (mM) of nitrogen source | Doubling time (min)
|
||
|---|---|---|---|---|
| Wild typeb | Δmep1 Δmep2 Δmep3b | Δvph1 | ||
| Glutamate | 10 | 160 | 160 | 230 |
| Proline | 10 | 200 | 200 | 410 |
| Arginine | 2.5 | 160 | 170 | 180 |
| Urea | 5 | 270 | 270 | 450 |
| NH4Cl | 20 | 160 | 175 | 205 |
| 5 | 160 | 290 | 210 | |
| 1 | 175 | >700 | 215 | |
a
Cells were grown in medium 164 with glucose (3%) as the carbon source and different nitrogen sources at the concentrations indicated. Strains were 23344c (wild type), 31019b (Δmep1 Δmep2 Δmep3) (35), and NCM3243 (Δvph1).
b
Final ODs of strains 23344c and 31019b were 13 and 13 on glutamate, 12 and 13 on proline, 11.5 and 11.5 on arginine, 14 and 13.5 on urea, 16 and 16 on 20 mM NH4Cl, 10 and 7 on 5 mM NH4Cl, and 1.3 and 0.4 on 1 mM NH4Cl.