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Effect of pH on the growth of S. cerevisiae at different ammonium concentrations
| (NH4+ + NH3) concentration (mM) | Doubling time (min) at pH:a
|
|||
|---|---|---|---|---|
| 5.5 | 6.5 | 7.5 | 8.0 | |
| 7 | 165 | 165 | 220 | 420b |
| 70 | 165 | 170 | 1,200b | NGc |
Cells were grown in medium 164 with glucose (3%) as the carbon source and ammonium as the sole nitrogen source. At pH values of 7.5 and 8.0, 50 mM Tris was added to the medium for buffering. The wild-type strain was 23344c.
Initial doubling time.
NG, no growth.
