TABLE 4.
Quantitative analysis of 14C-labeled products accumulated by N. crassa during transport of [14C]methylammonium
| Time after exposure (min)a | Normalized intensity (%) of indicated product accumulated by:b
|
|||||
|---|---|---|---|---|---|---|
| Wild type
|
vma-1
|
|||||
| CH3NH3+ | CH3-Gln | Origin | CH3NH3+ | CH3-Gln | Origin | |
| 1 | 93 | 2 | 5 | 92 | 4 | 4 |
| 4 | 87 | 4 | 9 | 77 | 16 | 7 |
| 10 | 80 | 8 | 12 | 52 | 37 | 11 |
| 25 | 60 | 17 | 23 | 18 | 57 | 25 |
a
Thin-layer chromatography plates were analyzed with a Molecular Dynamics PhosphorImager. Samples were as shown in Fig. 4B (see Materials and Methods). Strains were 74A (wild type) and pvn2-53-19A (vma-1) (4).
b
CH3NH3+, [14C]methylammonium; CH3-Gln, [14C]methylglutamine; origin, 14C-labeled products that were not resolved in our system.