FIG. 1.

Glycogen accumulation in snf1 pcl8 pcl10 cells with multicopy suppressor plasmids. Strains are EG328-1A (wild type [WT]) or ZWS2 (snf1 pcl8 pcl10). The plasmids are pRS425 (vector) or pYEP13 with the indicated gene inserted. In the screen, APG1 was found 17 times, APG13 was found 1 time, PDE2 was found 8 times, RCK2 was found 3 times, GAC1 was found 2 times, and YGR161C was found 2 times. The cells were grown on SD-Leu plates for 2 days at 30°C and then exposed to iodine vapor for 2 min to assess glycogen accumulation, with the darker the staining, the greater the glycogen accumulation.