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. 2022 Jan 5;10(1):e01664-21. doi: 10.1128/spectrum.01664-21

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Copyright © 2022 Van Moll et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 5 — Membrane activity of Hill-Cec1 and Hill-Cec10. (a) Outer membrane (OM) permeabilization of P. aeruginosa caused by Hill-Cec1 measured with N-phenyl-naphthylamine (NPN). (b) OM permeabilization of P. aeruginosa caused by Hill-Cec10 measured with NPN. NPN uptake is expressed as a percentage of the maximal uptake recorded with a high dose (16 μM) of polymyxin B. (c) Fluorescence caused by propidium iodide (PI) uptake in P. aeruginosa after addition of Hill-Cec1. (d) Fluorescence caused by PI uptake in P. aeruginosa after addition of Hill-Cec10. Values were normalized with the negative control. (e) Fluorescent signal of 3,3′-dipropylthiadicarbocyanine iodide (diSC₃(5)) as an indicator of cytoplasmic membrane depolarization of P. aeruginosa caused by Hill-Cec1. (f) Fluorescent signal of diSC₃(5) as an indicator of cytoplasmic membrane depolarization of P. aeruginosa caused by Hill-Cec10. Fluorescent signals were normalized with the negative control. AU, arbitrary units; S, start of measurement immediately after dye exposure.